Method

FEIA (Fluorescence Enzyme Immunoassay, ImmunoCAP (Solid-phase Immunofluorescence)) – quantitative determination.

Description

Pumpkin (Cucurbita) is an annual plant from the Cucurbitaceae family, widely distributed in temperate and tropical regions of the world. Pumpkin fruits are usually large, round, ribbed, and orange in color, although there are various shapes and varieties, including straw-colored and fine-textured types. Pumpkin is a valuable source of beta-carotene and vitamin E and is also used in cooking and traditional medicine. At the same time, pumpkin can cause allergic reactions in sensitive individuals, including dermatitis, asthma, itching, angioedema, and other symptoms. Cross-reactivity with other cucurbits such as melon, watermelon, cucumber, and zucchini is possible.

Indications

Diagnosis of allergic reactions to pumpkin.
Assessment of sensitization level and risk of allergy development.
Monitoring therapy effectiveness.
Evaluation of sensitization level after allergen avoidance.
Assessment of immune system status.

Preparation

Blood should be drawn in the morning on an empty stomach or 4 hours after eating.
Drinking plain still water is allowed.
Do not smoke for 30 minutes before the test.
For children under 1 year – no food 30-40 minutes before the test; for children 1-5 years – 2-3 hours fasting.

Clinical Significance

The test determines sensitization to pumpkin allergen, aiding in the diagnosis and treatment of allergic diseases. Since pumpkin shows cross-reactivity with other members of the cucurbit family (melon, watermelon, cucumber, zucchini), the results can be useful for comprehensive allergy risk assessment. The test is important for patients with food allergies and those suspected of latex-fruit syndrome, where cross-reactivity of proteins may occur.

Pumpkin (f225)0926

Specifications

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