Method

Immunofluorescence test (IFT) – quantitative determination of antineutrophil antibodies.

Description

Antineutrophil cytoplasmic antibodies (ANCA) play an important role in diagnosing various types of vasculitis, including Wegener's granulomatosis, microscopic polyangiitis, Churg-Strauss syndrome, and others. Perinuclear antineutrophil antibodies (pANCA), a subgroup of ANCA, are often detected in patients with inflammatory bowel diseases, particularly ulcerative colitis.

Indications

Granulomatosis with polyangiitis (early Wegener's granulomatosis)
Rapidly progressive glomerulonephritis
Vasculitis (panarteritis, microscopic polyarthritis, eosinophilic granulomatosis with polyangiitis)
Crohn's disease
Autoimmune liver diseases

Preparation

Blood collection in the morning on an empty stomach from 8 to 11 AM or 4-5 hours after the last meal.
Avoid fatty foods 24 hours before the test.
For children under 1 year – do not feed 30-40 minutes before the test.
For children 1-5 years old – refrain from eating 2-3 hours before the test.
Drink 1–2 glasses of still water before blood collection; for children, give water in portions of 150-200 ml over 30 minutes.
Blood collection should be done before taking medications; indicate medication intake in the referral.
Avoid physical exertion, stress, and do not smoke 30 minutes before the test.

Clinical Significance

Wegener's granulomatosis is a rare systemic disease with granulomatous-necrotizing vasculitis affecting the respiratory tract and lungs. Diagnosis is challenging due to nonspecific symptoms in early stages, often leading to delayed treatment and worsened prognosis. Determining pANCA helps timely diagnosis and monitoring of disease progression.

Perinuclear antineutrophil cytoplasmic antibodies (pANCA)0146

Specifications

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