Method

Determination of IgE antibodies to guinea pig epithelium by enzyme-linked immunosorbent assay (ELISA), which allows assessing the degree of sensitization of the body to allergens of this animal.

Description

The guinea pig epidermis consists of cells from the surface layer of the skin, which are one of the main allergens of this animal. Allergens are present in household and laboratory dust and can cause allergic reactions both through direct contact with the animal and by inhaling dust containing allergens. Allergy symptoms include bronchial asthma, allergic rhinitis, and conjunctivitis. Cross-reactivity between epidermal and urine allergens of the guinea pig is possible.

Indications

Diagnosis of allergic diseases: food allergy, atopic dermatitis, bronchial asthma, allergic rhinitis, respiratory allergy.
Assessment of the risk of developing allergic reactions to guinea pig epidermis.
Monitoring the effectiveness of drug treatment and allergen-specific immunotherapy (ASIT).

Preparation

Blood should be drawn in the morning on an empty stomach or 4 hours after eating.
Drinking plain still water is allowed.
Do not smoke for 30 minutes before the test.
For children under 1 year old – do not feed 30-40 minutes before the test; for children aged 1-5 years – 2-3 hours fasting.

Clinical Significance

Determining the presence and degree of sensitization to guinea pig epidermis helps diagnose allergic reactions manifesting as bronchial asthma, allergic rhinitis, conjunctivitis, and dermatitis. The test is important for patients who come into contact with guinea pigs, as well as for assessing allergy risk in laboratory professionals and pet owners.

Guinea pig epithelium (e6)1766

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