Method

HPLC (High-Performance Liquid Chromatography) – quantitative determination of vitamin C in blood serum.

Description

Vitamin C (ascorbic acid) is a water-soluble vitamin essential for many biological processes in the human body. It is not synthesized by the body and is stored in minimal amounts, so its reserves must be constantly replenished through diet. The main sources are plant-based foods: rose hips, black currants, citrus fruits, green vegetables (especially broccoli), tomatoes, potatoes, and peppers.

Vitamin C has a powerful antioxidant effect, neutralizing free radicals and protecting cells from oxidative stress. It is a coenzyme in many metabolic processes: collagen formation, carnitine biosynthesis, iron absorption, and the conversion of dopamine to norepinephrine. Vitamin C is important for wound healing, tissue growth, adrenal gland function, hormone and interferon secretion, as well as the metabolism of folic acid, tyrosine, and phenylalanine.

Vitamin C also plays a role in improving iron absorption, reducing cholesterol and blood pressure levels, and strengthening the immune system. Smoking, stress, and hemodialysis increase the body's need for vitamin C.

Indications

Bleeding and inflammation of the gums
Slow wound healing
Malabsorption syndrome

Preparation

Blood sampling in the morning on an empty stomach (8 hours after the last meal). Drinking plain still water is allowed. Do not smoke for 30 minutes before the test. For children under 1 year – no food 30-40 minutes before the test; for children 1-5 years – 2-3 hours fasting. If possible, avoid taking medications 12 hours before the test (in consultation with a doctor).

Clinical Significance

Vitamin C is necessary for collagen synthesis and maintaining the integrity of connective tissue. Deficiency causes hemorrhages, bleeding gums, joint pain, hair loss, dry skin, weakness, and emotional instability. In children, deficiency disrupts bone growth. Excess vitamin C may cause stomach upset, elevated liver enzymes, and increased oxalate levels in urine, which can lead to kidney stone formation.

Hypovitaminosis is often associated with malabsorption syndrome, alcoholism, or restricted diet. Measuring vitamin C levels helps diagnose deficiency and monitor therapy.

Determination of Vitamin C (ascorbic acid) level in blood serum0307

Reviews

Unfortunately, there are no reviews for this test yet!

Viewed tests

Contact Numbers:

Email: